Abstract
An actinomycetes isolate G-0299 obtained from a forest soil showed strong phytotoxic activity to Digitaria ciliaris. For the foliar application study, the culture filtrate of the isolate G-0299 showed strong herbicidal activity only to D. ciliaris among the 12 monocot and 5 dicot weed species. And herbicidal activity at a concentration of 500, 250, 125 and 62.5 μg mL-1 of culture filtrate was 100%, 98%, 70% and 40%, respectively. Phytotoxic symptoms of the culture filtrate by foliar application were desiccation and burn-down or bleaching of leaves and finally plant death. And then the herbicidal activity was exhibited only under the light condition. Also, chlorophyll loss of D. ciliaris leaf tissues in the light condition was much higher than in the dark condition and then chlorophyll content decreased 82%, 5%, respectively. In conclusion, our results suggest that soil bacteria, isolate G-0299 could be a good candidate for new bio-herbicide and provide a new lead molecule for a more unique herbicide.
Acknowledgements
This research has been performed as a project No SI1708(Development of crop protection agents leading the future market) and supported by the KOREA RESEARCH INSTITUTE of CHEMICAL TECHNOLOGY(KRICT).
Figures & Tables
Herbicidal activity of foliar application of the isolate G-0299 culture filtrate to five broad leaf and five grass weed species in a greenhouse condition. Herbicidal activity was determined by visual injury (0: no injury, 100: complete death) at 10 days after treatment. The isolate G-0299 effectively controlled only D. ciliaris among 10 weed species with foliar application of 2,000 µg mL concentration (red circle). The representative pictures were taken 10 days after foliar application.
