Synergistic Suppression of Bio-sulfur of Pencycuron on Large Patch Disease Caused by Rhizoctonia solani AG2-2 (IV) in Zoysiajaponica

Young Sun Kim1   JaePil  Lee2   Seog Won Chang3,*   Geung Joo Lee 1,*   

1Department of Horticultural Science, Chungnam National University, Daejeon 34134, Korea
2Golf industry Department, Konkuk University, Seooul 05029, Korea
3Department of Golf Course Management and Turfgrass Science Institute, Korea Golf University, Hoengseong 25247, Korea

Abstract

This study was conducted to evaluate effects of bio-sulfur on control of large patch disease caused by Rhizoctonia solani AG2-2 (IV) on Zoysiagrass (Zoysia japonica) in 2 regions (A site: Gangwon; B site: Gyeonggi). Treatments were as follows: Control (non-treatment), bio-sulfur treatment (BS), fungicide treatment (pencycuron; P), P+BS treatment, and 0.75P+BS treatment. During the experiments, no negative damage by BS or P treatment was found on turfgrass leaves. The bio-sulfur did not induce any antifungal activity against R. solani AG2-2 (IV) in vitro, or in vivo repression when applied without aeration and vertical mowing. Despite of non-significant effects of BS on large patch control, suppression efficiency against large patch in 0.75P+B combination was about 72-75%, which was similar or higher than that of P only treatment. When the BS or P treatment with aeration and vertical mowing in root zone of large patch infect area in 0.75P+B treatment was controlled about 70% in B site. These results indicated that bio-sulfur application only did not show antifungal activity against R. solani AG2-2 (IV) or control the large patch disease, but the combined application with pencycuron improved control efficiency as appeared by suppression of infect area, demonstrating that bio-sulfur might act as a synergist of the fungicide.

Figures & Tables

Fig. 1. In vitro test of antifungal activity of bio-sulfur (BS) against R. solani AG2-2 (IV). A: BS 10 fold; B: BS 100 fold; C: BS 1,000 fold.