Herbicidal Action Charateristics of Cellulose Biosynthesis Inhibitors against Giant Duckweed and Establishment of Novel Assay System

Jin-Seog Kim 1,*   Bo Gwan Kim 1   Hyeonju Noh 2   Ill Young  Lee 1   

1Research Center for Eco-Friendly New Materials, Bio & Drug Discovery Division, Korea Research Institute of Chemical Technology, Daejeon 34114, Korea
2Chemical Analysis Center, Chemical Platform Technology Division, Korea Research Institute of Chemical Technology, Daejeon 34114, Korea

Abstract

This study was conducted to find out the cellulose biosynthesis inhibitor (CBI)-specific symptoms in duckweeds (Lemnaceae), and to establish a novel “in vivo one-step screening method” using its symptoms as a criteria of CBI isolation. There were different between Spirodela polyrhiza (SPIPO) and Lemna paucicostata (LEMPA) in a responses to CBI. In SPIPO, the rapid detachment of fronds within one day after treatment and black-brown coloring/necrosis around active division part of frond would likely to be indicator symptoms of CBI that able to be distinguished from other mode of actions (HRAC/WSSA group 3, 14, 15, etc.) of herbicides. Black-brown coloring/necrosis of SPIPO fronds was enhanced by relatively high light and addition of glucose into medium. The black-brown pigments were not extracted by general solvents such as methanol and acetone. To know the qualitative characteristics of the black-brown pigments, FT-IR, UV-VIS, and DU-UV/VIS spectra were analyzed with the coloring frond tissues. RGB color analysis and Visual rating system were recommended to quantitatively evaluate the degree of black-brown coloring of tissue. Based on these results, a novel “in vivo one-step screening method” using SPIPO symptomology was established. It seemed to be a very useful means to rapidly screen and develop a new CBI with lower cost.

Figures & Tables

Fig. 1. Effect of several herbicides on the growth and black-brown coloring of at 5 d after treatment.