Herbicidal Effects and Component Analysis of Clove Oil and Pine Oil as a Plant Essential oils

Sung Geun Lee1   Soo Jeong Ahn2   Jeum Kyu Hong1   Sang Woo Lee1   Jae Gill Yun1   Kee Woong Park3   Sung Hwan Choi1,4,*   Jeung Joo Lee5,6,*   

1Department of Horticulture, Gyeongnam National University of Science and Technology, Jinju 52725, Korea
2Agricultural Corporation ERANG Co., Ltd., Changwon 51217, Korea
3Department of Crop Science, Chungnam National University, Daejeon 34134, Korea
4Research Center for Plant Protection, Gyeongnam National University of Science and Technology, Jinju 52725, Korea
5Department of Plant Medicine, College of Agriculture and Life Sciences, Gyeongsang National University, Jinju 52828, Korea
6Institute of Agriculture and Life Science (IALS), Gyeongsang National University, Jinju 52828, Korea

Abstract

This study was conducted to investigate the reduction of rice chlorophyll content and herbicidal effects of clove oil and pine oil, and to identify the components of major organic compounds contained in the plant essential oils. The chlorophyll content of rice after 24, 48, 96, and 168 hours of plant essential oil treatment was 59.9, 54.6, 27.1, and 14.5% in the case of 10 times dilution clove oil, and 47.2, 23.5, 15.7, and 10.9% in pine oil 10 times dilution, respectively. The chlorophyll reduction effect of 10 times dilution of clove oil and pine oil was greater than that of the recommended amount of glufosinate ammonium. As the concentration of the plant essential oil dilution increased, herbicidal effects increased, but the effect was different depending on the weed species. Herbicidal effects was higher in the glass weeds than in the broadleaf weeds, and the control effects on Amaranthus mangostanus and Bidens tripartita among the broadleaf weeds tended to be higher than those of Acalypha australis, Galinsoga ciliata, and Commelina communis. As a result of analyzing the components of essential plant oils, the main components of clove oil were caryophyllene (44.61%) belonging to squiterpene and eugenol (38.93%) belonging to phenolpropanoids, and the main components of pine oil were (+)-3-carene (38.53%), α-terpinolen (23.15%) and limonene (10.86%) belonging to monoterpene.

Figures & Tables

Fig. 1. Effects of essential oil from (clove oil) treatments on contents (%) of chlorophyll in leaves of rice in greenhouse condition. Above, ×160 and so on mean dilution folds. Bars on the graph is standard deviation. a-c: Means within a column followed by the same lowercase letters are not significantly different by Duncan's multiple range test at 5%.