Herbicidal Activity of KRA16-334 Broth Filtrate against Ragweed (Ambrosia artemisiifolia)

Jae-Deok Kim 1   Young-Sook Kim 1   Kyoung-Soo Jang1   He-Kyoung Lim 1   Kee Woong Park2   Lim Jeong-Hyun 3   Kyong-Mi Chon3   Jung-Sup Choi 1,*   

1Korea Research Institute of Chemical Technology, Daejeon 34114, Korea
2Chungnam National University, Daejeon 34134, Korea
3National Institute of Agricultural Sciences, RDA, Wanju 55365, Korea


Ambrosia artemisifilia (Rag weed) is one of the first ecosystem-disrupting plant designated by the Ministry of Environment, and it is found to be distributed in the largest area among ecosystem-disrupting plants in Korea. This study was conducted to control A. artemisiifilia with KRA16-334 broth filtrate. The broth filtrate of the KRA16-334 was applied to A. artemisiifilia in a greenhouse. The results showed that the foliar applications of KRA16-334 broth filtrate at all application rates demonstrated excellent herbicidal activity against the early (2-3 Ls), middle (4-5 Ls), and late (7-8 Ls) stages of A. artemisiifilia. Two times of KRA16-334 broth filtrate application suppressed regrwoth of A. artemisiifilia shoot. The main symptom of external manifestation was water soaked dots within 24 hours after treatment and continued to the appearance of chlorosis and proceeded to leaf burn-down, ultimately defoliations to dead plants. Our results show that the KRA16-334 broth filtrate can be utilized as a natural and eco-friendly herbicide against A. artemisiifilia that is one of the broad-leaved weeds disturbing Korean ecosystems.

Figures & Tables

Fig. 1. Herbicidal activity of foliar application of KRA16-334 culture broth filtrate to with early leaf stage (2-3Ls) in a greenhouse condition. Herbicidal activity was determined by visual injury (0: No injury, 100: Complete death). The representative pictures were taken 15 days after foliar application. DAT: Days after treatment.